Protective Effect of Grape Seed Extract on Gentamicin-Induced Acute Kidney Injury

Authors

  • Javid Safa Drug Applied Research Center and Department of Nephrology, Tabriz University of Medical Sciences, Tabriz, Iran Author
  • Hassan Argani Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz and Department of Nephrology, Shahid Beheshti University of Medical Sciences, Tehran, Iran Author
  • Bahar Bastani Division of Nephrology, Saint Louis University School of Medicine, Saint Louis, Missouri, USA Author
  • Nariman Nezami Drug Applied Research Center, Department of Nephrology, and Young Researchers Club, Tabriz University of Medical Sciences, Tabriz, Iran Author
  • Babak Rahimi Ardebili Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Author
  • Amir Ghorbanihaghjo Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Author
  • Hassan Kalagheichi Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Author
  • Akbar Amirfirouzi Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Author
  • Mehran Mesgari Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Author
  • Jafar Soleimany Rad Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Author

Abstract

Introduction. This study was designed to determine the protective effect of red grape seed extract (RGSE) on gentamicin-induced nephrotoxicity in rats.

Materials and Methods. Thirty male Wistar rats were divided into 3 groups to receive RGSE, for 60 days followed by intraperitoneal injection of saline solution (as placebo) for 8 days (group 1); RGSE followed by gentamicin for 8 days (group 2); and gentamicin without pre-medication of RGSE (group 3). Oral RGSE, 40 mg/kg/d, and intraperitoneal injection of gentamicin, 100 mg/kg/d, were administered in these groups of rats. Blood and urine samples were collected on days 0 and 68 of the study. Then, the kidneys were removed for pathologic examination.

Results. On day 68, serum creatinine and blood urea nitrogen concentrations were highest in group 3, which was significantly higher than in group 1 (P = .001 and P = .004, respectively), while slightly higher than in group 2 (P = .30 and P = .50, respectively). Fractional excretion of sodium was not significantly different between the three groups. Histopathological evaluation showed that rats in group 3 had significantly higher degrees of severe acute tubular necrosis and interstitial mononuclear cell infiltration than the rats in groups 1 and 2 (P < .001).

Conclusions. This animal study suggests that pretreatment with RGSE protects against gentamicin-induced acute kidney injury as evident on tissue histology. However, this was not accompanied with significant improvement in biochemical markers of kidney injury.

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Author Biography

  • Bahar Bastani, Division of Nephrology, Saint Louis University School of Medicine, Saint Louis, Missouri, USA

    Professor of Medicine - Nephrology

    Saint Louis University

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Published

2010-09-16

Issue

Section

ORIGINAL | Kidney Diseases

How to Cite

Protective Effect of Grape Seed Extract on Gentamicin-Induced Acute Kidney Injury. (2010). Iranian Journal of Kidney Diseases, 4(4), 285-291. https://ijkd.org/index.php/ijkd/article/view/227

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