Effects of Stem Cells and Granulocyte Colony Stimulating Factor in Reperfusion Injury

Authors

  • Mohsen Nafar Department of Nephrology, Shahid Labbafinejad Medical Center and Urology and Nephrology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran Author
  • Mahmoud Parvin Department of Pathology, Shahid Labbafinejad Medical Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran Author
  • Pejman Sadeghi Shahid Beheshti University of Medical Sciences, Tehran, Iran Author
  • Mohammed Ghoraishian Shahid Beheshti University of Medical Sciences, Tehran, Iran Author
  • Masoud Soleimani Department of Hematology, Tarbiat Modarres University, Tehran, Iran Author
  • Ali Tabibi Department of Urology, Shahid Labbafinejad Medical Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran Author
  • Akbar Nouralizadeh Department of Urology, Shahid Labbafinejad Medical Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran Author
  • Saeid Amirkhanlou Department of Nephrology, Shahid Labbafinejad Medical Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran Author
  • Farnaz Barzi Department of Nephrology, Tehran University of Medical Sciences, Tehran, Iran Author
  • Behrang Alipour Department of Nephrology, Shahid Labbafinejad Medical Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran Author

Abstract

Introduction. Bone marrow-derived stem cells have a potential capacity to differentiate and accelerate recovery in injured sites of body. Also, factors like granulocyte colony stimulating factor (GCSF) can promote their mobilization to the injured sites. We aimed to investigate the role of GCSF as an alternative therapeutic option instead of mesenchymal stem cells (MSCs) in reperfusion injury.

Materials and Methods. Twenty-nine rats with induced reperfusion injury were divided into 3 groups to receive MSC, GCSF, or nothing (control). Kidney function was assessed by blood urea nitrogen and serum creatinine levels. Histological grading was performed to evaluate the extent of tubular injury and the rate of recovery.

Results. All the rats reached recovery after 14 days. Rats in the MSC group reached early functional and histological recovery compared to the controls on the 7th day of the study (P = .01 and P = .02, respectively). Compared to the control group, the GCSF group showed a more significant histological recovery on the 7th day (P = .04), but kidney function was ameliorated on the 14th day (P = .04). Both the GCSF and control groups had a significant number of CD34+ cells, which were detected by flow cytometry on the 7th day after reperfusion injury.

Conclusions We found therapeutic effects following administration of both MSC and GCSF which was more evident with MSC in the setting of reperfusion injury. More investigation is required to find optimal time, dose, and route of administration as well as other possible contributing factors.

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Published

2010-06-16

Issue

Section

ORIGINAL | Kidney Diseases

How to Cite

Effects of Stem Cells and Granulocyte Colony Stimulating Factor in Reperfusion Injury. (2010). Iranian Journal of Kidney Diseases, 4(3), 207-213. https://ijkd.org/index.php/ijkd/article/view/379

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