The Protective Effects of Indole-Acetic Acid on the Renal Ischemia-Reperfusion Injury via Antioxidant and Antiapoptotic Properties in A Rat Model

Abstract

Introduction. The significant role of oxidative stress in the occurrence and development of a variety of diseases, including renal ischemia-reperfusion injury, has been thoroughly studied in this research. In this study, the protective role of indole-acetic acid on antioxidant, apoptotic and histopathological parameters in a rat model of renal ischemia-reperfusion (IR) injury were investigated. Methods. We divided 40 rats into the following four groups (n = 10 per group): healthy control, IR control, IR + indole-acetic acid 40 mg/kg, and IR + indole-acetic acid 60 mg/kg. After two weeks, the rats were anesthetized and their kidneys were removed. The effects of indole-acetic acid on biochemical parameters [glutathione peroxidase (GPx) and catalase (CAT) were measured by spectrophotometry and expression of apoptotic genes (BAX and Bcl2) using real-time RT-PCR. Tubular necrosis was evaluated using a histopathological study. Results. There were significant improvements in biochemical parameters (GPx), expression of the apoptotic genes (BAX) and tubular necrosis in rats treated with indole-acetic acid. Conclusion. Indole-acetic acid could reduce the effects of factors involved in the pathogenesis of IR, including oxidative stress, apoptosis and tubular necrosis. It can be recommended that, indoleacetic acid may be useful for amelioration of damages caused by IR.

DOI: 10.52547/ijkd.6894