Attenuating Renal Interstitial Fibrosis by Shenqi Pill via Reducing Inflammation Response Regulated by NF-κB Pathway In Vitro and In Vivo
DOI:
https://doi.org/10.52547/ggc27334Abstract
Introduction. One of the most significant clinical features of chronic
kidney disease is renal interstitial fibrosis (RIF). This study aimed
to investigate the role and mechanism of Shenqi Pill (SQP) on RIF.
Methods. RIF model was established by conducting unilateral
ureteral obstruction (UUO) surgery on rat or stimulating human
kidney-2 (HK-2) cell with transforming growth factor β1 (TGFβ1).
After modeling, the rats in the SQP low dose group (SQP-L), SQP
middle dose group (SQP-M) and SQP high dose group (SQP-H)
were treated with SQP at 1.5, 3 or 6 g/kg/d, and the cells in the
TGFβ1+SQP-L/M/H were treated with 2.5%, 5%, 10% SQP-containing
serum. In in vivo assays, serum creatinine (SCr) and blood urea
nitrogen (BUN) content were measured, kidney histopathology
was evaluated., and α-smooth muscle actin (α‐SMA) expression
was detected by immunohistochemistry. Interleukin-1β (IL-1β),
interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) content,
inhibitor of kappa B alpha (IKBα) and P65 phosphorylation were
assessed. Meanwhile, cell viability, inflammatory cytokines content,
α‐SMA expression, IKBα and P65 phosphorylation were detected
in vitro experiment.
Results. SQP exhibited reno-protective effect by decreasing SCr
and BUN content, improving renal interstitial damage, blunting
fibronectin (FN) and α‐SMA expression in RIF rats. Similarly, after
the treatment with SQP-containing serum, viability and α‐SMA
expression were remarkably decreased in TGFβ1-stimulated HK-2
cell. Furthermore, SQP markedly down-regulated IL-1β, IL-6, and
TNF-α content, IKBα and RelA (P65) phosphorylation both in vivo
and in vitro.
Conclusion. SQP has a reno-protective effect against RIF in vivo
and in vitro, and the effect is partly linked to nuclear factor-kappa
B (NF-κB) pathway related inflammatory response, which indicates
that SQP may be a candidate drug for RIF.
DOI: 10.52547/ijkd.7546
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