XPD expression and its effect on methylation on Benzene-induced blood toxicity
DOI:
https://doi.org/10.52547/vnz28561Keywords:
Benzene; Hydroquinone; Xeroderma pigmentosum complementation group D; methylationAbstract
Benzene is a known carcinogen that mostly targets and affects the hematopoietic system. It may cause a reduction of whole blood cells, aplastic anemia or even leukemia. To understand the molecular mechanisms of benzene-induced blood toxicity, the epigenetic changes of a deoxyribonucleic acid (DNA) repair gene, Xeroderma pigmentosum complementation group D (XPD), were investigated. Sprague-Dawley (SD) rats and K562 cells were exposed to different concentrations of benzene and its metabolite, hydroquinone. The results showed that both compounds may lead to DNA damage in a dose-dependent manner. Moreover, when their exposure reached a certain dose and time, the expression of XPD messenger ribonucleic acid (mRNA) and protein level increased. Measurement of the methylation status of the XPD gene promoter region in exposed cells revealed that the methylation rate of the XPD gene among groups did not differ significantly. The results of the present study suggested that the changes in XPD mRNA expression and protein level induced by exposure to benzene and its metabolite hydroquinone were not attributed to the abnormal methylation of the XPD gene.
Downloads
References
Martha S, Linet, Gilbert Ethel S, Vermeulen Roel, Dores Graça M, Yin Song-Nian, Portengen Lutzen, Hayes Richard B, Ji Bu-Tian, Lan Qing, Li Gui-Lan, and Rothman Nathaniel. "Benzene Exposure Response and Risk of Myeloid Neoplasms in Chinese Workers: A Multicenter Case-Cohort Study." J Natl Cancer Inst 111 (2018).
Damiano, Carbonari, Chiarella Pieranna, Mansi Antonella, Pigini Daniela, Iavicoli Sergio, and Tranfo Giovanna. "Biomarkers of Susceptibility Following Benzene Exposure: Influence of Genetic Polymorphisms on Benzene Metabolism and Health Effects." Biomark Med 10 (2016).
Martyn T, Smith, Zhang Luoping, McHale Cliona M, Skibola Christine F, and Rappaport Stephen M. "Benzene, the Exposome and Future Investigations of Leukemia Etiology." Chem Biol Interact 192 (2011).
Chen, Gao, Zhang Zhengbao, Chen Liping, and Chen Wen. "[the Advance of Model of Action in Low-Dose Chronic Benzene Exposure Induced Hematotoxicity]." Zhonghua Yu Fang Yi Xue Za Zhi 49 (2016).
Swaen, Gerard MH, Ludovic van Amelsvoort, Johannes J Twisk, Etienne Verstraeten, Ronald Slootweg, James J Collins, and Carol J Burns. "Low Level Occupational Benzene Exposure and Hematological Parameters." Chemico-biological interactions 184, no. 1-2 (2010): 94-100.
Tongshuai, Wang, Cao Yiyi, Xia Zhaolin, Christiani David C, and Au William W. "Review on Novel Toxicological Effects and Personalized Health Hazard in Workers Exposed to Low Doses of Benzene." Arch Toxicol 98 (2023).
Mengqi, Yan, Zhu Hongkai, Luo Haining, Zhang Tao, Sun Hongwen, and Kannan Kurunthachalam. "Daily Exposure to Environmental Volatile Organic Compounds Triggers Oxidative Damage: Evidence from a Large-Scale Survey in China." Environ Sci Technol 57 (2023).
Shen, Yang, Perret Vincent, Hager Jörin Corinne, Niculita-Hirzel Hélène, Goyette Pernot Joëlle, and Licina Dusan. "Volatile Organic Compounds in 169 Energy-Efficient Dwellings in Switzerland." Indoor Air 30 (2020).
Feier, Wang, Ye Lizhu, Jiang Xinhang, Zhang Rui, Chen Shen, Chen Liping, Yu Hongyao, Zeng Xiaowen, Li Daochuan, Xing Xiumei, Xiao Yongmei, and Chen Wen. "Specific Cpg Sites Methylation Is Associated with Hematotoxicity in Low-Dose Benzene-Exposed Workers." Environ Int 186 (2024).
Jiaqi, Li, Jiang Songfu, Chen Yi, Ma Ruye, Chen Jingjing, Qian Shanhu, Shi Yifen, Han Yixiang, Zhang Shenghui, and Yu Kang. "Benzene Metabolite Hydroquinone Induces Apoptosis of Bone Marrow Mononuclear Cells through Inhibition of β-Catenin Signaling." Toxicol In Vitro 46 (2017).
Yuting, Chen, Chen Jiajia, Yun Lin, Xu Longmei, Liu Jiaxian, Xu Yongchun, Yang Hui, Liang Hairong, and Tang Huanwen. "Hydroquinone-Induced Malignant Transformation of Tk6 Cells by Facilitating Sirt1-Mediated P53 Degradation and up-Regulating Kras." Toxicol Lett 259 (2016).
Lingxue, Yu, Qiu Weifeng, Gao Yuting, Sun Mingwei, Chen Lin, Cui Zheming, Zhu Delong, Guo Pu, Tang Huanwen, and Luo Hao. "Jnk1 Activated Prb/E2f1 and Inhibited P53/P21 Signaling Pathway Is Involved in Hydroquinone-Induced Pathway Malignant Transformation of Tk6 Cells by Accelerating the Cell Cycle Progression." Environ Toxicol 38 (2023).
Adriana-Stela, Crișan, Tripon Florin, Bogliș Alina, Crauciuc George-Andrei, Trifa Adrian P, Lázár Erzsébet, Macarie Ioan, Gabor Manuela Rozalia, and Bănescu Claudia. "The Role of DNA Repair (Xpc, Xpd, Xpf, and Xpg) Gene Polymorphisms in the Development of Myeloproliferative Neoplasms." Medicina (Kaunas) 60 (2024).
Jinseok, Kim, Li Chia-Lung, Chen Xuemin, Cui Yanxiang, Golebiowski Filip M, Wang Huaibin, Hanaoka Fumio, Sugasawa Kaoru, and Yang Wei. "Lesion Recognition by Xpc, Tfiih and Xpa in DNA Excision Repair." Nature 617 (2023).
Hiba, Hassanain, Tseitline Dana, Hacohen Tamar, Yifrach Adi, Kirshenbaum Ayala, Lavi Bar, Parnas Avital, and Adar Sheera. "A Practical Site-Specific Method for the Detection of Bulky DNA Damages." J Mol Biol 436 (2024).
Yumei, Tian, Lin Xiaojuan, Yang Fan, Zhao Jitong, Yao Kui, and Bian Ce. "Contribution of Xeroderma Pigmentosum Complementation Group D Gene Polymorphisms in Breast and Ovarian Cancer Susceptibility: A Protocol for Systematic Review and Meta Analysis." Medicine (Baltimore) 99 (2020).
Bang-Shun, He, Xu Tao, Pan Yu-Qin, Wang Han-Jin, Cho William C, Lin Kang, Sun Hui-Ling, Gao Tian-Yi, and Wang Shu-Kui. "Nucleotide Excision Repair Pathway Gene Polymorphisms Are Linked to Breast Cancer Risk in a Chinese Population." Oncotarget 7 (2016).
Mengyun, Wang, Li Qiaoxin, Gu Chengyuan, Zhu Yao, Yang Yajun, Wang Jiucun, Jin Li, He Jing, Ye Dingwei, and Wei Qingyi. "Polymorphisms in Nucleotide Excision Repair Genes and Risk of Primary Prostate Cancer in Chinese Han Populations." Oncotarget 8 (2016).
Hua, W, X Han, F Li, L Lu, Y Sun, H Hassanian-Moghaddam, M Tian, Y Lu, and Q Huang. "Transgenerational Effects of Arsenic Exposure on Learning and Memory in Rats: Crosstalk between Arsenic Methylation, Hippocampal Metabolism, and Histone Modifications." Environmental science & technology 58, no. 15 (2024): 6475-86.
Kang, YT, WJ Yang, HC Huang, SC Tang, and JL Ko. "Exposure to Nickel Chloride Induces Epigenetic Modification on Detoxification Enzyme Glutathione S-Transferase M2." Environmental toxicology 39, no. 3 (2024): 1729-36.
Iyer, M, U Anand, S Thiruvenkataswamy, HWS Babu, A Narayanasamy, VK Prajapati, CK Tiwari, AV Gopalakrishnan, E Bontempi, C Sonne, D Barceló, and B Vellingiri. "A Review of Chromium (Cr) Epigenetic Toxicity and Health Hazards." The Science of the total environment 882 (2023): 163483.
Weidong, Ji, Yang Linqing, Yu Lei, Yuan Jianhui, Hu Dalin, Zhang Wenjuan, Yang Jianping, Pang Yaqin, Li Wenxue, Lu Jiachun, Fu Juan, Chen Jiakun, Lin Zhongning, Chen Wen, and Zhuang Zhixiong. "Epigenetic Silencing of O6-Methylguanine DNA Methyltransferase Gene in Nis-Transformed Cells." Carcinogenesis 29 (2008).
Jing, Yang, Zuo Xin, Bai Wenlin, Niu Piye, Tian Lin, and Gao Ai. "Pten Methylation Involved in Benzene-Induced Hematotoxicity." Exp Mol Pathol 96 (2014).
S A, Bustin, Benes V, Nolan T, and Pfaffl M W. "Quantitative Real-Time Rt-Pcr--a Perspective." J Mol Endocrinol 34 (2005).
Xiaohan, Yang, Li Chao, Yu Gongchang, Sun Lei, Guo Sumei, Sai Linlin, Bo Cunxiang, Xing Caihong, Shao Hua, Peng Cheng, and Jia Qiang. "Ligand-Independent Activation of Ahr by Hydroquinone Mediates Benzene-Induced Hematopoietic Toxicity." Chem Biol Interact 355 (2022).
Yan, Sha, Yang Zhenyu, Zhou Wei, Zhu Xiaoling, and Xiang Yingping. "[Role of Poly (Adp-Ribose) Polymerase 1 in DNA Methylation Changes Induced by Hydroquinone in Human Bronchial Epithelial Cell]." Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 32 (2014).
Bogdanović, Ozren, and Gert Jan C Veenstra. "DNA Methylation and Methyl-Cpg Binding Proteins: Developmental Requirements and Function." Chromosoma 118 (2009): 549-65.
Kristi A, Koelsch, Webb Ryan, Jeffries Matlock, Dozmorov Mikhail G, Frank Mark Barton, Guthridge Joel M, James Judith A, Wren Jonathan D, and Sawalha Amr H. "Functional Characterization of the Mecp2/Irak1 Lupus Risk Haplotype in Human T Cells and a Human Mecp2 Transgenic Mouse." J Autoimmun 41 (2013).
Xu, Ting, Hongmei Ding, Jie Chen, Jiahui Lei, Meng Zhao, Bingyu Ji, Youguo Chen, Songbing Qin, and Qinqin Gao. "Research Progress of DNA Methylation in Endometrial Cancer." Biomolecules 12, no. 7 (2022): 938.
Haiqiao, Zhang, Yuan Qian, Pan Zhijie, Ling Xiaoxuan, Tan Qiang, Wu Minhua, Zheng Dongyan, Xie Peien, Xie Daxiao, and Liu Linhua. "Up-Regulation of Dnmt3b Contributes to Hotairm1 Silencing Via DNA Hypermethylation in Cells Transformed by Long-Term Exposure to Hydroquinone and Workers Exposed to Benzene." Toxicol Lett 322 (2020).
Anastasia, Conti, Rota Federica, Ragni Enrico, Favero Chiara, Motta Valeria, Lazzari Lorenza, Bollati Valentina, Fustinoni Silvia, and Dieci Giorgio. "Hydroquinone Induces DNA Hypomethylation-Independent Overexpression of Retroelements in Human Leukemia and Hematopoietic Stem Cells." Biochem Biophys Res Commun 474 (2016).
Nicola A, Philbrook, and Winn Louise M. "Investigating the Effects of in Utero Benzene Exposure on Epigenetic Modifications in Maternal and Fetal Cd-1 Mice." Toxicol Appl Pharmacol 289 (2015).
Valentina, Bollati, Baccarelli Andrea, Hou Lifang, Bonzini Matteo, Fustinoni Silvia, Cavallo Domenico, Byun Hyang-Min, Jiang Jiayi, Marinelli Barbara, Pesatori Angela C, Bertazzi Pier A, and Yang Allen S. "Changes in DNA Methylation Patterns in Subjects Exposed to Low-Dose Benzene." Cancer Res 67 (2007).
Carless, Melanie. "Investigation of Genomic Methylation Status Using Methylation-Specific and Bisulfite Sequencing Polymerase Chain Reaction." Chromatin Protocols: Second Edition (2009): 217-34.
Rikio, Suzuki, Onizuka Makoto, Kojima Minoru, Shimada Masako, Fukagawa Satomi, Tsuboi Kosuke, Kobayashi Hiroyuki, Shintani Ayumi, Ogawa Yoshiaki, Kawada Hiroshi, Hotta Tomomitsu, and Ando Kiyoshi. "Preferential Hypermethylation of the Dickkopf-1 Promoter in Core-Binding Factor Leukaemia." Br J Haematol 138 (2007).
Li, Xiao-Yu, Jian-Zhen Shen, Song-Fei Shen, Hai-Ying Fu, Hua-Rong Zhou, Dan-Sen Wu, Yuan-Yuan Zhang, and Yong-Qing Zheng. "Methylation Status of Iex-1 Gene Promotor Cpg Island in Malignant Hematopoietic Cell Lines." Zhongguo shi yan xue ye xue za zhi 19, no. 2 (2011): 473-76.
Wei Jie, Seow, Pesatori Angela Cecilia, Dimont Emmanuel, Farmer Peter B, Albetti Benedetta, Ettinger Adrienne S, Bollati Valentina, Bolognesi Claudia, Roggieri Paola, Panev Teodor I, Georgieva Tzveta, Merlo Domenico Franco, Bertazzi Pier Alberto, and Baccarelli Andrea A. "Urinary Benzene Biomarkers and DNA Methylation in Bulgarian Petrochemical Workers: Study Findings and Comparison of Linear and Beta Regression Models." PLoS One 7 (2012).
Downloads
Published
Data Availability Statement
none
